CLINICAL SIGNIFICANCE
Brucella diagnostic may be assessed either by microorganism isolation in blood or stools, or by titration of specific antibodies in the patient serum.The reagent, because of its formulation in an acid buffer, is reactive with both IgG and IgM antibodies and very useful for the diagnosis of chronic individuals, which present a high level of IgG antibody, difficult to be detected by the reference tube method (Wright).
PRINCIPLE
The Rose Bengal is a slide agglutination test for the qualitative and semiquantitative detection of antibodies anti-Brucella in human serum. The stained bacterial suspension agglutinates when mixed with samples containing specific IgG or IgM antibodies present in the patient sample.